Details:

• Alternative Title:
  Sci Transl Med
• Personal Author:
  Agarwal, Kavita ; Choudhury, Biswa ; Robinson, Lloyd S. ; Morrill, Sydney R. ; Bouchibiti, Yasmine ; ... More +

  Agarwal, Kavita ; Choudhury, Biswa ; Robinson, Lloyd S. ; Morrill, Sydney R. ; Bouchibiti, Yasmine ; Chilin-Fuentes, Daisy ; Rosenthal, Sara B. ; Fisch, Kathleen M. ; Peipert, Jeffrey F. ; Lebrilla, Carlito B. ; Allsworth, Jenifer E. ; Lewis, Amanda L. ; Lewis, Warren G. Less -
• Description:
  Epithelial cells are covered in carbohydrates (glycans). This glycan coat or "glycocalyx" interfaces directly with microbes, providing a protective barrier against potential pathogens. Bacterial vaginosis (BV) is a condition associated with adverse health outcomes in which bacteria reside in direct proximity to the vaginal epithelium. Some of these bacteria, including |, produce glycosyl hydrolase enzymes. However, glycans of the human vaginal epithelial surface have not been studied in detail. Here, we elucidate key characteristics of the "normal" vaginal epithelial glycan landscape and analyze the impact of resident microbes on the surface glycocalyx. In human BV, glycocalyx staining was visibly diminished in electron micrographs compared to controls. Biochemical and mass spectrometric analysis showed that, compared to normal vaginal epithelial cells, BV cells were depleted of sialylated |- and |-glycans, with underlying galactose residues exposed on the surface. Treatment of primary epithelial cells from BV-negative women with recombinant | sialidases generated BV-like glycan phenotypes. Exposure of cultured VK2 vaginal epithelial cells to recombinant | sialidase led to desialylation of glycans and induction of pathways regulating cell death, differentiation, and inflammatory responses. These data provide evidence that vaginal epithelial cells exhibit an altered glycan landscape in BV and suggest that BV-associated glycosidic enzymes may lead to changes in epithelial gene transcription that promote cell turnover and regulate responses toward the resident microbiome.
• Subjects:
  [+]

  Bacteria Female Gardnerella Vaginalis Humans Neuraminidase Polysaccharides Vagina Vaginosis, Bacterial
• Keywords:
  [+]

  Alzheimer's Disease Alzheimer's Disease Sequencing Project Antiviral Agents Herpes Simplex Human Papillomavirus Torque Teno Viruses Whole Exome Sequencing Whole Genome Sequencing
• Source:
  Sci Transl Med. 15(724):eabp9599
• Pubmed ID:
  38019934
• Pubmed Central ID:
  PMC11419735
• Document Type:
  Journal Article
• Funding:
  UL1 TR001442/TR/NCATS NIH HHSUnited States/ ; S10 OD026929/CD/ODCDC CDC HHSUnited States/ ; R01 AI127554/AI/NIAID NIH HHSUnited States/ ; S10 OD026929/OD/NIH HHSUnited States/ ; R01 AI114635/AI/NIAID NIH HHSUnited States/

  UL1 TR001442/TR/NCATS NIH HHSUnited States/ ; S10 OD026929/CD/ODCDC CDC HHSUnited States/ ; R01 AI127554/AI/NIAID NIH HHSUnited States/ ; S10 OD026929/OD/NIH HHSUnited States/ ; R01 AI114635/AI/NIAID NIH HHSUnited States/ Less -
• Volume:
  15
• Issue:
  724
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  [+]

  urn:sha-512:d1e08f749a6b942339fba12dacdb7cc3fb21709f25ee99e690fb7fa1b282022ebd54e9a8ae5a93f9cf55b2abd306c3f21f298c417a430df3fa9085fa3ef7f80f
• File Type:

• Supporting Files:
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